Molecular Diagnostics & Therapeutics
Experimental Therapeutics Laboratory
Our laboratory is interested in developing new mechanism-based drugs for the treatment of gastrointestinal cancers including esophageal, colon and pancreatic cancers and genital cancers (prostate and ovarian) and pediatric malignancies, leukemia, medulloblastoma and neurobalstoma. We are working on a new structural class of compounds that can target selective Sp protein transcription factors and survivin. We are also working on novel combination therapies for enhancing the response of radiation or chemotherapy in pancreatic, ovarian and pediatric malignancies.
Publications
(in chronological order)
Sankpal, U.T., Maliakal, P., Bose, D., Kayaleh, O. R., Buchholz, D., and Basha, R. Expression of Specificity Protein Transcription Factors in Pancreatic Cancer and their Association in Diagnosis, Prognosis and Therapeutic Target. Curr. Med. Chem. In press.
Sankpal, U.T., Pius, H., Khan, M., Shukoor, M.I., Maliakal, P., Lee, C.M., Abdelrahim, M., Connelly, S.F., and Basha, R. Environmental Factors in Causing Human Cancers: Emphasis on Tumorigenesis. Tumor Biology. In press.
Sankpal, U.T, Abdelrahim, M., Connelly, S.F., Lee, C.M., Madero-visbal, R., Colon, J., C.H., Smith, J., Safe, S., Malikal, P., and Basha, R. (2012). Small molecule tolfenamic acid inhibits PC-3 cell proliferation and invasion in vitro, and tumor growth in orthotopic mouse model for prostate cancer. The Prostate. [Epub ahead of print]. PMID: 22473873.
Eslin D., Sankpal, U.T., Chris Lee, Sutphin, R., Maliakal, P., Currier, E., Sholler, G., and Basha, R. Tolfenamic acid inhibits cell proliferation and induces cell apoptosis in neuroblastoma cell lines: a novel therapeutic agent for neuroblastoma. Mol. Carcinogenesis. Epub ahead of print. Dec., 2011. PMID: 22213339.
Rahim, A., Nafi-valencia, E., Siddiqi, S., Basha, R., Runyon, C.C., Siddiqi, S.A. (2012). Proteomic Analysis of the Very Low Density Lipoprotein (VLDL) Transport Vesicles. J Proteomics. 75(7):2225-35. PMID: 22449872.
Maliakal, P., Abdelrahim, M., Sankpal, U.T., Baker, C.H., Herrera, L., Safe, S., Abudayyeh, A, S. Kaja, and Basha, M.R. (2012). Chemopreventive effects of tolfenamic acid against esophageal tumorigenesis in rats. Invest New Drugs. 30(3):853-61. PMID: 21197621.
Basha, R. (2011). Contemporary Approaches in Cancer Therapy. Med Chem. 7(5): 430-431. PMID: 22022993.
Sankpal, U.T., Goodison, S., Abdelrahim, M., and Basha, R. (2011). Targeting SP1 transcription factors in prostate cancer therapy. Med Chem. 7(5): 518-525. PMID: 22022994.
Sankpal, U.T., Kaja, S., Rahim, A., Patel, P., Abdelrahim, M., and Basha, R. (2011). Role of environment in human cancers. In: Environment and Health. Eds. Reddy, G.R., Flora, S.J.S., Basha, R., Narosa Publishing house, New Delhi. pp 136-147.
Maliakal, P., Sankpal, U.T., Basha, R., Maliakal, C., Ledford A., and Wanwimolruk, S. (2011). Relevance of drug metabolizing enzymes activity modulation by tea polyphenols in the inhibition of esophageal tumorigenesis Med Chem. 7(5): 480-487. PMID: 21801144.
Adwan, L.I., Basha, R., Abdelrahim, M., Zawia, N.H. (2011). Tolfenamic acid interrupts the de novo synthesis of the amyloid precursor protein and lowers amyloid beta via a transcriptional pathway. Curr Alzheimer Res. 8(4): 385-892. PMID: 21557719.
Basha, R., Ingersoll, S.B., Sankpal, U.T, Ahmad, S., Baker, C.H., Edwards, J.R., Holloway, R.W., Kaja, S., and Abdelrahim, M. (2011). Tolfenamic Acid Inhibits Ovarian cancer cells growth and decreases the expression of c-Met and survivin through suppressing the transcription factors Sp1 and Sp3. Gyn Oncology. 122(1): 163-70. PMID: 21496890.
Basha, R., Baker, C.H., Sankpal, U., Ahmad, S., Safe, S., Abdelrahim, M. (2011). Therapeutic Applications of Non-Steroidal Anti-Inflammatory Drugs in Cancer: Special Emphasis on Tolfenamic Acid. Front Biosci (Schol Ed). 3: 797-805. PMID: 21196413.
Colon, J., Basha, M.R., Madero-Visbal, R., Konduri, S., Baker, C.H., Herrera, L.J., Safe, S., Sheikh-Hamad, D., Abudayyeh, A., Alvarado, B., Abdelrahim, M. (2011). Tolfenamic acid decreases c-Met expression through Sp proteins degradation and inhibits lung cancer cells growth and tumor formation in orthotopic mice. Invest. New Drugs, 29(1):41-51. PMID: 19851711.
Basha, R., Reddy, G.R. (2010). Developmental exposure to lead and late life abnormalities of the nervous system. Indian J Exp Biol. 48: 636-41. PMID: 20929048.
Aziz, N., Jha, A., Thanos, C., Basha, R, Bose, A. (2010). Structural markers in prostate cancer serum imaged ex vivo using cryogenic transmission electron microscopy. J Electron Microsc (Tokyo). 59(5): 451-6. PMID: 20445004.
Jutooru, I, Chadalapaka, G., Abdelrahim, M., Basha, M.R., Samudio, I., Konopleva, M., Andreeff, M, and Safe, S. (2010). Methyl 2-Cyano-3,12-dioxooleana-1,9-dien-28-oate (CDDO-Me) Decreases Specificity Protein (Sp) Transcription Factors and Inhibits Pancreatic Tumor Growth. Mol Pharmcol. 78(2): 226-36. PMID: 20488920.
Zawia, N.H., Basha, R. (2010). Developmental lead exposure epigenetics and late onset of Alzheimer’s disease. In: Developmental Neurotoxicology Research, (Eds. Wang, C., Slikker, W.), pp 143-62, John Wiley & Sons Inc., Hoboken, NJ, USA.
Abdelrahim, M., Konduri, S., Basha, M.R., Abudayyeh, A., Baker, C.H. (2010). Angiogenesis: A recent update and potential drug approaches. Int. J. Oncol. 36: 5-18. PMID: 19956828.
Konduri, S., Colon, J., Baker, C.H., Safe S., Abbruzzese, J.L, Abudayyeh, A., Basha, M.R., and Abdelrahim, M. (2009). Tolfenamic acid enhances pancreatic cancer cell and tumor response to radiation therapy by inhibiting survivin protein expression. Mol. Cancer Ther. 8(3): 533-542. PMID:19258429.
Basha, M.R., Baker, C.H., Abdelrahim, M. (2009). Biomarkers clinical relevance in cancer: Emphasis on breast cancer and prostate cancer. Curr. Trends Biotechnol. Pharmacy 3(1): 1-7.
Dosunmu, R., Wu, J., Adwan, L., Maloney, B., Basha, M.R., McPherson, C.A., Harry, J.G., Rice, D.C., Zawia, N.H., Lahiri, D.K. (2009). Lifespan profiles of AD associated genes and their products in monkeys and mice. J. Alzheimers Dis. 18(1): 211-230. PMID: 19584442.
Wu, J., Basha, M.R., Brock, B., Cox, D.P., Cardozo-Pelaez, F., McPherson, C.A., Harry, J., Rice, D.C., Maloney, B., Chen, D., Lahiri, D.K, Zawia. N.H. (2008). Alzheimer's disease (AD)-like pathology in aged monkeys after infantile exposure to environmental metal lead (Pb): Evidence for a developmental origin and environmental link for AD. J. Neurosci. 28(1): 3-9. PMID: 18171917.
Brock, B., Basha, R., DiPalma, K., Anderson, A., Harry, G.J., Rice, D.C,, Maloney, B., Lahiri, D.K, Zawia, N.H. (2008). Co-localization and distribution of cerebral APP and SP1 and its relationship to amyloidogenesis. J. Alzheimers Dis. 13(1): 71-80. PMID: 18334759.
Lahiri, D.K., Maloney, B., Basha, M.R., Ge, Y.W., Zawia, N.H. (2007). How and when environmental agents and dietary factors affect the course of Alzheimer’s disease: The “LEARn” model (Latent Early Associated Regulation) may explain the triggering of AD. Curr. Alzheimer’s Res. 4(2): 219-228. PMID: 17430250.
Dosunmu R, Wu, J., Basha M.R., Zawia, N.H. (2007). Environmental and dietary risk factors in Alzheimer’s disease: An overview. Expert Rev. Neurotherapeutics 7: 887-900. PMID: 17610395.
White, L.D., Cory-Slechta, D.A., Gilbert, M.E., Tiffany-Castiglioni, E., Zawia, N.H., Virgolini, M., Rossi-George, A., Lasley, S.M., Qian, Y., Basha, M.R. (2007). New and evolving concepts in the neurotoxicology of lead. Toxicol. Appl. Pharmacol. 225(1): 1-27. PMID: 17904601.
Wu, J., Basha, M.R., Zawia, N.H. (2007). The environment, epigenetics and amyloidogenesis. J. Mol. Neurosci. 34(1): 1-7. PMID: 18157652.
Bakheet, S.A., Basha, M.R., Zawia, N.H. (2007). Lead exposure: expression and functionality of oct-2 in the developing rat brain. Toxicol. Sci. 95(2): 436-442. PMID: 17093203.
Bolin, C.S., Basha, M.R., Cox, D., Zawia, N.H., Maloney, B., Lahiri, D. K., Cardozo-Pelaez, F. (2006). Exposure to lead (Pb) and the developmental origin of oxidative DNA damage in the aging brain. FASEB J. 20(6): 788-790. PMID: 16484331.
Basha, M.R., Braddy, N.S., Zawia, N.H., Kodavanti, P.R.S. (2006). Ontogenetic alterations in certain nuclear transcription factors in cerebellum and hippocampus following perinatal exposure to commercial PCB mixture. NeuroToxicology 27: 118-124. PMID: 16159668.
Zawia, N.H., Basha, M.R. (2005). Environmental risk factors and developmental basis for Alzheimer's disease. Rev. Neurosci. 16: 325-337. PMID: 16519009.
Basha, M.R., Murali, M., Siddiqi, H.K., Ghosal, K., Siddiqi, O.K., Lashuel, H.A., Ge, Y.W., Lahiri, D.K., Zawia, N.H. (2005). Exposure to lead (Pb) promotes ß-Amyloid aggregation but does not affect the proteolytic processing of amyloid precursor protein. FASEB J. 19(14): 2083-2084. PMID: 16230335.
Basha, M.R., Wei, W., Bakheet, S.A., Benitez, N., Siddiqi, H.K., Ge, Y., Lahiri, D.K., Zawia, N.H. (2005). The fetal-basis of amyloidogenesis: Exposure to lead and latent over expression of amyloid precursor protein and ß-amyloid in the aging brain. J. Neuroscience, 25(4): 823-829. PMID: 15673661.
Basha, M.R., Wei, W., Reddy, G.R., Zawia, N.H. (2004). Zinc finger transcription factors mediate perturbations of brain gene expression elicited by heavy metals. In: Molecular Neurotoxicology Environmental Agents and Transcription-Transduction Coupling, (Ed. N. H. Zawia), pp. 43-64, CRC Press, USA
Zawia, N.H., Basha, M.R., Razmiafshari, M. (2003). An electrophoretic method to determine the ability of xenobiotic metals to bind a specific protein domain. Curr. Protocols Toxicol. 12(11): 1-8.
Basha, M.R., Wei, W., Brydie, M, Razmiafshari, M., Zawia, N.H. (2003). Lead induced developmental perturbations in hippocampal Sp1 DNA-binding are prevented by Zinc. In vivo evidence for Pb and Zinc competition. Int. J. Dev. Neurosci. 21(1): 1-12. PMID: 12565691.
Atkins, D.S., Basha, M.R., Zawia, N.H. (2003). Intracellular signaling pathways involved in mediating the effects of lead on the transcription factor Sp1. Int. J. Dev. Neurosci. 21: 235-244. PMID: 12850056.
Reddy, G.R., Basha, M.R., Bhuvaneswari, C., Suresh, A., Baker, J.L., Shafeek, S.A., Heinz, J., Chetty, C.S. (2003). Lead-induced effects on acetyl cholinesterase activity in cerebellum and hippocampus of developing rat. Int. J. Dev. Neurosci. 21(6): 347-352. PMID: 12927583.
Basha, M.R., Wei, W., Zawia, N.H. (2003). PCBs and environmental metal Pb exacerbate Ab aggregation and its cytotoxicity in PC12 cells. Ogranohalogen Compounds 65: 12-15.
Basha, M.R., Brady, N.S., Kodavanti, P.S., Zawia, N.H. (2002). Changes in the DNA-binding of several transcription factors in the developing rat cerebellum by PCBs. Ogranohalogen Compounds 57: 381-383.