Molecular Diagnostics & Therapeutics
Tumor Metastasis Laboratory
Dr. Urquidi’s research interests are primarily cancer research, and include molecular pathology, the function of specific genes in the metastatic spread of cancer, and biomarker discovery and development for clinical diagnosis and prognosis.
Ongoing research projects include the elucidation of mechanisms of metastasis as a basis for the development of new and better approaches to the prevention and treatment of metastases in breast and other cancer types. This is achieved through derivation of models, application of proteomic and genomic molecular profiling techniques, and functional analysis of genes with the potential to alter the metastatic propensity of breast and prostate cancer cells.
Additional interests include developing tools for the early detection of bladder cancer, and for the individualized assessment of prognosis for treated cancer patients.
Principal-investigator:
Co-investigarors:
Publications
Sun, Y., Urquidi, V. and Goodison, S. (2010) Derivation of molecular signatures for breast cancer recurrence prediction using a two-way validation approach. Breast Cancer Res Treat, 119, 593-9.
Goodison, S., Sun, Y. and Urquidi, V. (2010) Derivation of cancer diagnostic and prognostic signatures from gene expression data. Bioanalysis, 2, 855-62.
Sakai, Y., Goodison, S., Cao, W., Urquidi, V., Namiki, K., Porvasnik, S., Urbanek, C. and Rosser, C.J. (2009) VEGF induces expression of Bcl-2 and multiple signaling factors in microvascular endothelial cells in a prostate cancer model. World J Urol, 27, 659-66.
Goodison, S., Rosser, C.J. and Urquidi, V. (2009) Urinary proteomic profiling for diagnostic bladder cancer biomarkers. Expert Rev Proteomics, 6, 507-14.
Goodison, S. and Urquidi, V. (2008) Breast tumor metastasis: analysis via proteomic profiling. Expert Rev Proteomics, 5, 457-67.
Urquidi, V. and Goodison, S. (2007) Genomic signatures of breast cancer metastasis. Cytogenet Genome Res, 118, 116-29.
Kreunin, P., Yoo, C., Urquidi, V., Lubman, D.M. and Goodison, S. (2007) Proteomic profiling identifies breast tumor metastasis-associated factors in an isogenic model. Proteomics, 7, 299-312.
Kreunin, P., Yoo, C., Urquidi, V., Lubman, D.M. and Goodison, S. (2007) Differential expression of ribosomal proteins in a human metastasis model identified by coupling 2-D liquid chromatography and mass spectrometry. Cancer Genomics Proteomics, 4, 329-39.
Goodison, S., Yuan, J., Sloan, D., Kim, R., Li, C., Popescu, N.C. and Urquidi, V. (2005) The RhoGAP protein DLC-1 functions as a metastasis suppressor in breast cancer cells. Cancer Res, 65, 6042-53.
Goodison, S., Viars, C. and Urquidi, V. (2005) Molecular cytogenetic analysis of a human breast metastasis model: identification of phenotype-specific chromosomal rearrangements. Cancer Genet Cytogenet, 156, 37-48.
Sloan, D.D., Nicholson, B., Urquidi, V. and Goodison, S. (2004) Detection of differentially expressed genes in an isogenic breast metastasis model using RNA arbitrarily primed-polymerase chain reaction coupled with array hybridization (RAP-array). Am J Pathol, 164, 315-23.
Kreunin, P., Urquidi, V., Lubman, D.M. and Goodison, S. (2004) Identification of metastasis-associated proteins in a human tumor metastasis model using the mass-mapping technique. Proteomics, 4, 2754-65.
Wang-Rodriguez, J., Urquidi, V., Rivard, A. and Goodison, S. (2003) Elevated osteopontin and thrombospondin expression identifies malignant human breast carcinoma but is not indicative of metastatic status. Breast Cancer Res, 5, R136-43.
Ma, H., Urquidi, V., Wong, J., Kleeman, J. and Goodison, S. (2003) Telomerase reverse transcriptase promoter regulation during myogenic differentiation of human RD rhabdomyosarcoma cells. Mol Cancer Res, 1, 739-46.
Hayashi, K., Goodison, S., Urquidi, V., Tarin, D., Lotan, R. and Tahara, E. (2003) Differential effects of retinoic acid on the growth of isogenic metastatic and non-metastatic breast cancer cell lines and their association with distinct expression of retinoic acid receptor beta isoforms 2 and 4. Int J Oncol, 22, 623-9.
Goodison, S., Viars, C., Grazzini, M. and Urquidi, V. (2003) The interrelationship between DRIM gene expression and cytogenetic and phenotypic characteristics in human breast tumor cell lines. BMC Genomics, 4, 39.
Goodison, S., Kawai, K., Hihara, J., Jiang, P., Yang, M., Urquidi, V., Hoffman, R.M. and Tarin, D. (2003) Prolonged dormancy and site-specific growth potential of cancer cells spontaneously disseminated from nonmetastatic breast tumors as revealed by labeling with green fluorescent protein. Clin Cancer Res, 9, 3808-14.
Urquidi, V., Sloan, D., Kawai, K., Agarwal, D., Woodman, A.C., Tarin, D. and Goodison, S. (2002) Contrasting expression of thrombospondin-1 and osteopontin correlates with absence or presence of metastatic phenotype in an isogenic model of spontaneous human breast cancer metastasis. Clin Cancer Res, 8, 61-74.
Kawai, K., Viars, C., Arden, K., Tarin, D., Urquidi, V. and Goodison, S. (2002) Comprehensive karyotyping of the HT-29 colon adenocarcinoma cell line. Genes Chromosomes Cancer, 34, 1-8.
Taniyama, K., Goodison, S., Ito, R., Bookstein, R., Miyoshi, N., Tahara, E., Tarin, D. and Urquidi, V. (2001) PTEN expression is maintained in sporadic colorectal tumours. J Pathol, 194, 341-8.
Urquidi, V., Tarin, D. and Goodison, S. (2000) Role of telomerase in cell senescence and oncogenesis. Annu Rev Med, 51, 65-79.
Idriss, H., Urquidi, V. and Basavappa, S. (2000) Selective modulation of P-glycoprotein's ATPase and anion efflux regulation activities with PKC alpha and PKC epsilon in Sf9 cells. Cancer Chemother Pharmacol, 46, 287-92.
Aogi, K., Woodman, A., Urquidi, V., Mangham, D.C., Tarin, D. and Goodison, S. (2000) Telomerase activity in soft-tissue and bone sarcomas. Clin Cancer Res, 6, 4776-81.
Goodison, S., Urquidi, V. and Tarin, D. (1999) CD44 cell adhesion molecules. Mol Pathol, 52, 189-96.
Aogi, K., Kitahara, K., Urquidi, V., Tarin, D. and Goodison, S. (1999) Comparison of telomerase and CD44 expression as diagnostic tumor markers in lesions of the thyroid. Clin Cancer Res, 5, 2790-7.
Urquidi, V. and Ashcroft, S.J. (1997) Molecular cloning of the cDNA encoding beta-cell calcium/calmodulin-dependent protein kinase II. Adv Exp Med Biol, 426, 91-6.
Tian, Y.M., Urquidi, V. and Ashcroft, S.J. (1996) Protein kinase C in beta-cells: expression of multiple isoforms and involvement in cholinergic stimulation of insulin secretion. Mol Cell Endocrinol, 119, 185-93.
Urquidi, V. and Ashcroft, S.J. (1995) A novel pancreatic beta-cell isoform of calcium/calmodulin-dependent protein kinase II (beta 3 isoform) contains a proline-rich tandem repeat in the association domain. FEBS Lett, 358, 23-6.
Urquidi, V. and Bishop, D.H. (1992) Non-random reassortment between the tripartite RNA genomes of La Crosse and snowshoe hare viruses. J Gen Virol, 73 ( Pt 9), 2255-65.
Urquidi, V. (1989) Molecular epidemiology of human rotavirus infection in Coro, Venezuela. Acta Cient Venez, 40, 33-9.
Nolan, K.F., Urquidi, V., Emery, V.C. and Bishop, D.H. (1989) The design and use of specific genetic probes to identify closely related
bunyaviruses and to determine the genotype of their recombinants. J Gen Virol, 70 ( Pt 8), 2201-7.
Urquidi, V. and Furzan, J. (1988) Comparison of rotavirus infection in newborn infants and hospitalized older children by electrophoresis of genome RNA. Acta Cient Venez, 39, 96-8.
Urquidi, V., Novo, E. and Esparza, J. (1981) Protein synthesis in cells infected with bovine rotavirus. J Gen Virol, 53, 363-9.